Interspecific hybrids between Lilium 'Oriental' hybrid and L. 'Asiatic' hybrid produced by embryo culture with revised media.

Interspeci®c hybrids between Lilium nobilissimum and L. regale produced via ovules-with-placental-tissue culture

Reciprocal pollination was made between Lilium nobilissimum and L. regale. Pollen tubes reached the base of style within 144 h after pollination, but no mature seeds were obtained in either cross combination. Explants, or ovules-with-placental-tissue excised from each carpel 30 and 40 days after pollination (DAP), were cultured on a medium composed of major salts of B-5 macronutrient (Gamborg, ...

Effect of a New Compound on the Mitotic Polyploidization of Lilium Longiflorum and Oriental Hybrid Lilies

A new compound, Surflan (Surflan), for mitotic polyploidization in vitro in lily was tested and compared with the original compound ‘oryzalin’. Surflan was effective for mitotic polyploidization of lilies. The optimal concentration of Surflan was 0.0075% for in vitro chromosome doubling. A low concentration (0.0025%) was still effective to produce polyploid plants, however, a high concentration...

Hybrids and hybrid zones

Determination by GISH and FISH of hybrid status in Lilium.

In the genus Lilium, plants obtained from crosses, especially between distant relatives, are not always hybrids because embryos can develop as a result of apomixis. These plants constitute genetic material of the maternal parent only. In this study, verification of hybrid status of plants which have been obtained from the crosses 'Marco Polo'xLilium henryi and 'Expression'xL. henryi was perform...

In situ embryo rescue for generation of wide intra‐ and interspecific hybrids of Panicum virgatum L.

Wide crosses have been used for decades as a method for transferring novel genetic material and traits in plant breeding. Historically, many products of wide crosses require tedious and inefficient surgical embryo rescue prior to embryo abortion to recover single plantlets. We have utilized transgenic switchgrass (Panicum virgatum L. cv Alamo) as a pollen donor in conjunction with antibiotic or...